Foundation Cell and Mol Biology

Foundation Cell and Mol Biology

 

LAB 10 WORKSHEET

 

1. Briefly describe the purpose of working with embryoless seeds. (15 points)

 

2. GA is a hormone that functions to? (10 points)

 

3. What is the role of Ca2+ ions in this context? (10 points)

 

4. Which groups are your:Positive control,Negative control, and experimental group? (15 points)

 

5. Using the data provided below which was obtained using 20ul of enzyme sample and incubated for 30seconds in a total volume of 1ml :

 

1. Calculate the UA for all the samples ( 20 points)
2. Build a semi log graph by plotting log of [GA] on the x-axis and the UAs on the y-axis (15 points)
3. Use this plot as a standard curve to calculate the [GA] in tube #15 which had an absorbance value of 0.5. (15 points)

 

[GA]	Abs 625
0.05	0.652
0.1	0.53
0.5	0.504
1	0.396
5	0.249

 

UA=(0.7-Abs625)*Volume of supernatant (ml) / incubation time (min)* enzyme extract volume (ml)

 

Hint:

Volume of supernatant= 1ml

Incubation time= 0.5 min

Enzyme extract volume = 0.02 ml

 

 

 

 

 

Foundation Cell and Mol Biology: Quiz 4

Grading: 1point for each question (No partial point)

Duration: 15minutes

Submission: Blackboard on grade center, any quiz sent by email will be deleted

(BB submission is the solo proof of your attendance)

 

1. Briefly describe the purpose of lab 9 : Molecular Biology of Sickle cell anemia.

 

 

1. Variant forms of a given gene are known as _____________.

 

1. What is the purpose of native gel electrophoresis?

 

1. On what basis are proteins separated in lab 9? Choose the correct answer.

1. Charge
2. Mass

 

5. a. In the image given below of your data, which sample seems to have moved fastest? Explain why that has happened, by applying what you learnt about the nature of those isoforms of Haemoglobin.

 

HbA/HbA

HbA/HbS

HbS/HbS

+ anode

 

1. Why did the sample in the middle form an elongated band that is moving intermediately?

 

 

 

1. The third band seems to be moving the slowest. Why did that happen?

 

 

6. What is the condition known as for a person with these genotypes:

1. HbA/HbS: ____________________
2. HbS/HbS: ____________________